RESEARCH FIELDChemistry › Inorganic chemistry
RESEARCHER PROFILEFirst Stage Researcher (R1)
APPLICATION DEADLINE15/04/2020 23:00 - Europe/Brussels
LOCATIONFrance › Grenoble
TYPE OF CONTRACTTemporary
HOURS PER WEEK35
OFFER STARTING DATE01/10/2020
PHD grant in bioinorganic chemistry
FIGHTING OXIDATIVE STRESS THANKS TO NISOD MIMICS
Funded by the Chemistry Biology Health graduate school at Univ. Grenoble Alpes
The superoxide radical anion, O2°-, is generated by many life processes including aerobic metabolism and photosynthesis. Although it has a positive role when its toxicity is used by the immune system to kill invading pathogens, O2°- is the precursor of most other Reactive Oxygen Species (ROS), such as hydrogen peroxide and hydroxyl radicals, which have deleterious consequences when not properly balanced by protective pathways. Cells submitted to oxidative stress are protected from superoxide by the superoxide dismutases (SODs), which catalyze the disproportionation of superoxide into hydrogen peroxide and oxygen.
This PhD project aims at developing innovative active NiSOD mimics with a pluridisciplinary approach combining peptide design, coordination and physical chemistry, theoretical modeling and cellular assays. It will be performed within a collaboration between two laboratories in Grenoble: SyMMES (UMR 5819 Univ. Grenoble Alpes, CEA, CNRS, supervisor Pascale Delangle) and DCM (UMR 5250, Univ. Grenoble Alpes, CNRS, supervisor Carole Duboc). NiSOD mimics based on a constrained peptide scaffold will be synthesized and fully characterized with complementary analytical and spectroscopic techniques. The determination of structure – SOD activity relationships in water with allow optimizing the peptide scaffolds to afford efficient SOD like catalysts, active in water and displaying antioxidant properties in cellular models relevant to oxidative stress diseases for therapeutic applications. The solubility of the Ni-SOD mimics will be tuned to allow studying intermediate species in organic solvents. Indeed stabilizing key catalytic species requires working at low temperature in organic solvents to avoid interfering protons. Coupling experimental and theoretical investigations will strongly contribute to full understanding of the catalytic mechanism of the NiSOD.
The PhD student recruited will be involved in all the steps of the project ensuring a pluridisciplinary formation. This will be also an opportunity to develop project management skills as he (or she) will interact with both laboratories and permanent staffs specialized in each part of the scientific work.
Profile of the candidate
The candidate should have a master’s degree in chemistry most preferably in bioinorganic chemistry or chemical biology. Good knowledge of analytical and spectroscopic methods used to characterize inorganic complexes will be a strong asset.
His/her motivation to do a PhD in general and more precisely for the proposed subject, as well as his/her ability to interact in a synergistic and constructive way with the people involved in the two laboratories, SyMMES and DCM, will also be key-elements for the selection of candidates.
To apply, please send a detailed resume, a cover letter describing how you would fit the requested profile, L3, M1 and M2 university results including grades and rankings and two reference letters to Pascale DELANGLE (email@example.com) before April 15th 2020.
Position available as of October 1st 2020.
EURAXESS offer ID: 508776
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